Assessment of sea urchin spawning induction and oocyte conservation applied to ecotoxicological assays
نویسندگان
چکیده
منابع مشابه
Sperm-oocyte interaction in the sea-urchin.
A study was made of the electrical and morphological changes in the sea-urchin oocyte during interaction with spermatozoa. The first event, a small step depolarization accompanied by a 20-40% decay in input resistance occurs within seconds of attachment. The evidence suggests that this electrical event is the result of sperm-oocyte fusion, and that the ion channels that lower the resistance acr...
متن کاملSpawning events in small and large populations of the green sea urchin Strongylocentrotus droebachiensis as recorded using fertilization assays
During the winter and spring of 2002 and 2003, we used time-integrated fertilization assays to monitor sperm availability in three populations of the green sea urchin Strongylocentrotus droebachiensis in Maine: a naturally occurring population of .40,000 urchins and two smaller groups (,1,000) of transplanted urchins isolated from other aggregations. Episodes of sperm release coincided in two p...
متن کاملCyclin B synthesis is required for sea urchin oocyte maturation.
Sea urchins are members of a limited group of animals in which meiotic maturation of oocytes is completed prior to fertilization. This is different from oocytes of most animals such as mammals and amphibians in which fertilization reactivates an arrested meiotic cycle. Using a recently developed technique for in vitro maturation of sea urchin oocytes, we analyzed the role of cyclin B, the regul...
متن کاملRegulatory contribution of heterotrimeric G-proteins to oocyte maturation in the sea urchin
Regulation of animal oocyte maturation is hypothesized to involve heterotrimeric G-proteins. It is difficult to test this hypothesis though without knowing what G-proteins are present in these cells and where are they localized. We set out to test the hypothesis that G-proteins regulate maturation in the sea urchin oocyte by identifying resident G-proteins in oocytes and eggs, and then investig...
متن کاملGene Regulatory Network Interactions in Sea Urchin Endomesoderm Induction
A major goal of contemporary studies of embryonic development is to understand large sets of regulatory changes that accompany the phenomenon of embryonic induction. The highly resolved sea urchin pregastrular endomesoderm-gene regulatory network (EM-GRN) provides a unique framework to study the global regulatory interactions underlying endomesoderm induction. Vegetal micromeres of the sea urch...
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ژورنال
عنوان ژورنال: Ocean and Coastal Research
سال: 2020
ISSN: 2675-2824
DOI: 10.1590/s2675-28242020068288